Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles
نویسندگان
چکیده
Here we report a novel method for the synthesis of glucosamine-functionalized gold nanoparticles (GlcN-AuNPs) using biocompatible and biodegradable glucosamine for antibacterial activity. GlcN-AuNPs were prepared using different concentrations of glucosamine. The synthesized AuNPs were characterized for surface plasmon resonance, surface morphology, fluorescence spectroscopy, and antibacterial activity. The minimum inhibitory concentrations (MICs) of the AuNPs, GlcN-AuNPs, and GlcN-AuNPs when irradiated by ultraviolet light and laser were investigated and compared with the MIC of standard kanamycin using Escherichia coli by the microdilution method. Laser-irradiated GlcN-AuNPs exhibited significant bactericidal activity against E. coli. Flow cytometry and fluorescence microscopic analysis supported the cell death mechanism in the presence of GlcN-AuNP-treated bacteria. Further, morphological changes in E. coli after laser treatment were investigated using atomic force microscopy and transmission electron microscopy. The overall results of this study suggest that the prepared nanoparticles have potential as a potent antibacterial agent for the treatment of a wide range of disease-causing bacteria.
منابع مشابه
Antibacterial response of Cd-TiO2/PEG/folic acid nanocomposite under ultraviolet, visible light, or ultrasonic irradiation
Synthesis and characterization of Cd-TiO2/PEG/FA nanocomposite as a biomaterial is the main aim of this research. Cd-doped TiO2 nanoparticles (NPs) were synthesized by solvothermal assisted sol-gel method. Then, polyethylene glycol (PEG) was added to the as-synthesized NPs in order to modify their surface and to prevent agglomeration. In the next step, folic acid (FA) was conjugated to Cd-TiO2/...
متن کاملManganese mine actinobacterial mediated gold nanoparticles synthesis and their antibacterial activity
Background and Objectives: Actinobacteria efficiently can produce different nanoparticles with different biological properties due to their ability to produce secondary metabolites. The aim of present study, isolation and screening of gold nanoparticles via producing actinobacteria from the soil were studied and their antibacterial activities was evaluate. Methods: In this study, after iso...
متن کاملSynthesis and Characterization of Gold Nanoparticles using Plant Extract of Terminalia arjuna with Antibacterial Activity
The use of plant extracts for nanoparticles synthesis are green, economical and cost effective approach. The present study reports the bio-synthesis of gold nanoparticles (Au NPs) using leaf extract of Terminalia arjuna. After exposing the gold ions to aqueous solution of leaf extract, rapid reduction of gold ions into gold nanoparticles is observed within few minutes. The characterizat...
متن کاملGold-Curcumin Nanostructure in Photo-thermal Therapy on Breast Cancer Cell Line: 650 and 808 nm Diode Lasers as Light Sources
Background: Au nanoparticles (AuNPs) exhibit very unique physiochemical and optical properties, which now are extensively studied in a range of medical diagnostic and therapeutic applications. AuNPs can be used for cancer clinical treatment with minimal invasion. On the other hand, curcumin is a polyphenol derived from turmeric which is used for medical purposes due to its anti...
متن کاملParameters Affecting the Biosynthesis of Gold Nanoparticles Using the Aquatic Extract of Scrophularia striata and their Antibacterial Properties
Green synthesis is a simple, low-cost, non-toxic, environmentally friendly and efficient approach touse. Leaf extract of plants rich in polyphenols, such as flavonoids, is a powerful agent in reducing thesynthesis of gold nanoparticles. The purpose of this study is to investigate the parameters affecting thebiosynthesis of gold nanoparticles using the aqueous extract of Scroph...
متن کامل